Somerville Elks Lodge 1068

Ambient Mass Spectrometry Imaging: Recent Developments and Opportunities Julia Laskin* Department of Chemistry, Purdue University, West Lafayette, IN, United States Mass spectrometry imaging (MSI) is a powerful technique for studying the localization of lipids, metabolites, and proteins in biological samples. We have developed nanospray desorption electrospray ionization (nano-DESI), an ambient ionization technique that enables label-free molecular imaging of biological samples with minimal sample pretreatment. Nano-DESI relies on the localized liquid extraction of analyte molecules from the sample into a liquid bridge formed between two glass capillaries. The extracted analytes are transferred to a mass spectrometer inlet and ionized by electrospray ionization. Quantification is performed by adding an internal standard to the solvent and normalizing the signals of endogenous analytes to the corresponding adduct of the standard. Recent developments of nano-DESI MSI instrumentation have enabled quantitative imaging of lipids, metabolites, and proteins in tissues with high sensitivity and spatial resolution down to 8-10 microns using finely pulled capillaries. Furthermore, we have developed a microfluidic nano-DESI probe, which greatly simplifies the experimental setup and demonstrates similar performance to the capillary-based probe. We have enhanced the chemical specificity of nano-DESI MSI experiments by coupling it with ion mobility spectrometry and online photochemical derivatization

The NJ Mass Spectrometry Discussion Group is pleased to announce our June 18, 2024 in-person Meeting, sponsored by Biognosys 5:30 PM    Social and Registration 6:15 PM    Complimentary Dinner 6:55 PM    Welcome and Opening Remarks 7:05 PM  Speaker 1:   Todd Hembrough, PhD, CEO and Co-Founder Nexosome Oncology, Identification of early-stage NSCLC biomarkers from extracellular vesicle microenvironment identifies tumor and immune response biomarkers associated with cancer, Abstract 8:00 PM Speaker 2:   Valesca Anshau, Scientific Director for North America for Biognosys;  Unveils Enhanced Plasma Proteomics for High-Throughput Biomarker Discovery, Abstract

The NJ Mass Spectrometry Discussion Group is pleased to announce our May 2, 2024 in-person Meeting. 5:30 PM    Social and Registration 6:15 PM    Complimentary Dinner 6:55 PM    Welcome and Opening Remarks 7:05 PM  Speaker 1:   Kallol Gupta, Nanobiology Institute, Yale University, and Department of Cell Biology, Yale School of Medicine, A nanoscale spatially resolved mass spectrometry-based platform for macromolecular cartography of the cellular membrane 8:00 PM Speaker 2:   Jared B. Shaw, Department of Chemistry, University of Nebraska-Lincoln;  New approaches for top-down and native top-down protein characterization Sponsored by NJACS MSDG with partial funding from ASMS Somerville Elks Lodge 1068 375 Union Avenue Bridgewater, 08807 United States+ Google Map View Venue Website

The NJ Mass Spectrometry Discussion Group is pleased to announce our April 9 2024 in-person Meeting. Program 5:30 PM    Social and Registration 6:15 PM    Complimentary Dinner 6:55 PM    Welcome and Opening Remarks 7:05 PM  Speaker 1: Darshit Shah, HLA-peptide discovery to develop tumor-specific immunotherapies for the treatment of cancer. Regeneron Pharmaceuticals 8:00 PM Speaker 2: Shourjo Ghose, Ultra-high sensitive Single Cell Proteomics on the timsTOF Ultra Attendance if Free Sponsored by Bruker